Facility

Animal House facility B.S.R.C. "Alexander Fleming"

The animal house unit of provides husbandry of animals and services to the biomedical research community since early 2001. The present facility has a capacity to house more than 18000 mice and comprises breeding colonies, stock and experimental mice including many transgenic and knockout mouse lines. Our animal house unit provides its services to the various research groups of the B.S.R. C. "Al.Fleming" as well as to external contractors in Europe and U.S.A. ( pharmaceutical and biotechnology companies, leading hospitals, and academic institutions).

Contact Persons:

M. Kamber

Address

Αλεξάνδρου Φλέμιγκ 34

Telephone

+30-210-965-6310 ext. 231

Fax

+30-210-965-6563

E-mail

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Webpage

www.animalfacility.eu

Facility

Transgenic facility                    B.S.R.C. "Alexander Fleming"

The Facility generates transgenic mice via:

• Pronuclear Injection-Founder Generation
• Embryonic Stem Cell Gene Targeting-Blastocyst Injection-Chimera Production- Germline transmission

In addition the facility facilitates the transportation, storage and archiving of mutant strains, via embryo rederivation, and cryopreservation and recovery of mouse embryo and sperm.

The Facility benefits from the research environment of BSRC “Al.Fleming” to provide an excellent service in transgenic technologies. The Facility had a track record of  successful projects with investigators within the BSRC Al. Fleming, collaborating academic institutions in Greece, Europe and USA, and biotech/pharmaceutical companies within Europe.

Contact Persons:

D. L. Kontoyiannis & amp; M. Alexiou

Address

34, Al. Fleming Str, 16672, Vari, Greece

Telephone

00302109654335

00302109656310 Ext. 251

Fax

00302109654955

E-mail

Kontoyiannis@fleming.gr

Alexiou@fleming.gr

Webpage

http://www.fleming.gr/tggt/

Facility

Laboratory of Macromolecular Analysis

 B.S.R.C. "Alexander Fleming"

The Laboratory of macromolecular analysis provides a core facility for the analysis and characterization of proteins and their interactions with other biomolecules. A strong emphasis has been given on the acquisition of state-of-the-art specialized instruments. A modern ion-trap mass spectrometer is available (Finnigan LCQ-DECA), providing high sensitivity and accuracy. Apart from the standard electrospray source, we have obtained a nanospray source, which is essential for the analysis of peptides at the low levels obtained from 2-D gels. Peptides are separated with a specialized nano- HPLC system (LC Packings UltiMate), using flow rates at the nanoliter range and very small diameter columns. All the necessary equipment for reproducible 2-D gels are available. A major instrument of the facility is a Surface Plasmon Resonance biosensor (BIAcore 3000) for the analysis of macromolecular interactions, an area where our group has extensive experience. An analytical HPLC system, as well as two FPLC systems for protein purification are also available.

Contact Persons:

G. Panayotou

M. Samiotaki

Address

34 Alexander Fleming, 16672, Vari, Greece

Telephone

+30-210-965-5054

+30-210-965-6310 (ext.193)

Fax

+30-210-9653934

E-mail

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Webpage

www.fleming.gr

Facility

Flow Cytometry facility                   B.S.R.C. "Alexander Fleming"

The Flow Cytometry Facility at BSRC’s Institute of Immunology provides investigators with support for flow cytometric analysis and operator-based cell sorting. The facility is equipped with flow cytometric analyzers capable for up to 8-colour analysis and high-throughput applications as well as a cell-sorting unit. Its personnel offers maintenance of the instruments, assistance on instrument usage, experimental design depending upon the researcher's knowledge and requirements, experimental troubleshooting and running of cell sorting experiments.

Contact Persons:

D.L. Kontoyiannis & S.Grammenoudi

Address

34, Al. Fleming Str., Vari 16672

Telephone

00302109654335

00302109656310 Ext. 218

Fax

00302109654955

E-mail

Kontoyiannis@fleming.gr

Grammenoudi@fleming.gr

Webpage

http://www.fleming.gr/en/services/flow/index.html

Facility

Expression Profiling Unit                    B.S.R.C. "Alexander Fleming"

The Expression Profiling Unit provides researchers with support in genome based research. Unit services are offered not only to academic and research centers, but the private sector as well, in the context of academic collaborations, participation in research networks and/or contracted services.

The Unit is equipped with the most advanced technology on microarray experimentation, including the use of an Affymetrix Scanner for high-throughput microarray experiments as well as all the equipment necessary for the generation of good quality results. A cDNA confocal scanner is also available for imaging and data analysis and a Luminex system for multiplex cytokine experiments. A complete and thorough data analysis is also conducted in order to generate and support customers with ready to publish results.

Contact Persons:

Vassilis Aidinis, PhD

Address

34 Al. Fleming Street, 16672 Vari, Greece

Telephone

+30 210 9654382

Fax

+30 210 9654210

E-mail

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Webpage

www.expressionprofiling.gr

Facility

BioIT unit                    B.S.R.C. "Alexander Fleming"

BioIT unit is concerned with data acquisition and integration from wet lab scientists both inside and outside the institute. Unit members focus on building, maintaining and providing biological databases and information services for the research community. Excellency of data curation and appliance of cutting edge technology have placed the unit in the centre of recent developments in the field of biological resources.

Contact Persons:

Vassilis Aidinis, PhD

Address

34 Al. Fleming Street, 16672 Vari, Greece

Telephone

+30 210 9654382

Fax

+30 210 9654210

E-mail

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Webpage

http://bioit.fleming.gr